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利用亨氏厌氧技术从沤麻塘底泥等厌氧生境中分离筛选到N2，Y4，Z2-6三株厌氧细菌，研究结果表明，其产酶最适pH为7．0－7．4，最适温度为30℃，最佳碳源为淀粉，最佳氮源为豆饼粉。N2、Y4、Z2-6的果胶酶活分别为：913u，973u，927U，半纤维素酶活分别为：137u、140u、170u，纤维素酶活分别为0、3u、0，对数生长期均为5－9小时，产酶高峰时间均在72小时左右。三个菌株混合发酵红麻皮，5天可成浆，粗浆得率为74．4％，半纤维素脱除率为28．79％，果胶脱除率为72．41％，脂蜡质脱除率为23．23％，打浆后做手抄片，白度为40．5，裂断长为3529m。对N2，Y4，Z2-6进行了初步鉴定，经查《伯杰细菌鉴定手册》第八版，三个菌株均属梭菌属的群Ⅲ。
The anaerobic bacteria N2, Y4 and Z2-6 were isolated and screened from anaerobic habitats such as Sediment pond sediment using Heinz’s anaerobic technique. The results showed that the optimum pH value for producing anaerobic bacteria was 7.0-7.4 , The optimum temperature was 30 ℃, the best carbon source was starch, the best nitrogen source was soybean meal. The pectinase activities of N2, Y4 and Z2-6 were 913u, 973u and 927U, respectively. The hemicellulase activities were 137u, 140u and 170u respectively, and the cellulase activities were 0, 3u and 0 respectively. The logarithmic growth The average period of 5-9 hours, peak enzyme production in about 72 hours. The three strains mixed fermented kenaf, five days can be pulp, coarse pulp yield was 74.4%, hemicellulose removal rate was 28.79%, pectin removal rate was 72.41%, fat wax The mass removal rate was 23.23%. After beating, a hand sheet was made with a whiteness of 40.5 and a rupture length of 3529 m. The initial identification of N2, Y4, Z2-6, the investigation of “Berger bacteria Identification Manual” eighth edition, the three strains are Clostridium group Ⅲ.