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目的:通过研究比较不同种质和表型的当归与地方习用品东当归、牡丹叶当归的差异,建立当归与东当归、牡丹叶当归之间的分子鉴别。方法:对14个不同种质和表型的当归及其地方习惯用品东当归、牡丹叶当归的ITS序列进行测序和分析,探讨该片段在中药当归分子生物学水平上的鉴定意义。结果:测得当归14个个体的ITS-1,5.8S rDNA和ITS-2全序列及18S rRNA基因3′端和26S rRNA基因5′端部分碱基序列,共约664bp,其中ITS1序列长度215bp,5.8S长162 bp,ITS2序列长度为223bp。当归与东当归的遗传距离为8.6,与牡丹叶当归的遗传距离为9.6,而东当归与牡丹叶当归的遗传距离为10.7。结论:从ITS序列结果来看,两种表型的绿茎当归和紫茎当归ITS序列并无区别,栽培和野生的当归其ITS序列也完全相同。用ITS区序列来对不同种质不同表型的当归的遗传多样性进行分析并不适宜。但高度保守的ITS区可以用来鉴定当归与东当归、牡丹叶当归。
OBJECTIVE: To establish the molecular identification between Angelica sinensis and Angelica sinensis and Angelica dahurica by comparing the differences between Angelica sinensis and Angelica sinensis with different local germplasm and geographical habitats, Angelica sinensis and Angelica sinensis. Methods: ITS sequences of Angelica sinensis and its local habitat Angelica sinensis and its tree peony Angelica sinensis from 14 different germplasm and phenotypes were sequenced and analyzed, and the significance of this fragment in the molecular biology of Angelica sinensis was discussed. Results: The ITS-1, 5.8S rDNA and ITS-2 sequences and the 5 ’end sequences of 18S rRNA gene and 26S rRNA gene of 14 individuals from Angelica sinensis were detected. The total length of ITS1 was 215bp 5.8S was 162 bp in length and ITS2 sequence was 223 bp in length. Angelica and Angelica sinensis genetic distance of 8.6, and Peony Angelica genetic distance was 9.6, and Angelica sinensis and Peony Angelica genetic distance was 10.7. Conclusion: From the results of ITS sequences, there is no difference between the ITS sequences of Angelica fortunei and Angelica Sinensis in the two phenotypes, and ITS sequences of cultivated and wild Angelica are also identical. It is not appropriate to use the ITS region sequences to analyze the genetic diversity of Angelica sinensis with different phenotypes of different germplasms. But the highly conserved ITS region can be used to identify Angelica sinensis and Angelica sinensis, Angelica sinensis.