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目的 建立军团菌rcp毒力基因聚合酶链反应(PCR)检测方法.方法 根据GenBanK公布的嗜肺军团菌核苷酸序列合成军团菌rcp毒力基因的特异引物,采用PCR方法对2005年-2007年天津市中央空调冷却塔分离的16株嗜肺军团菌、杜氏军团菌(Legionella dumoffii,L.dum)、博氏军团菌(Legionella bozemanii,L.boz)、长滩军团菌(Legionellafeeleii,L.fee)等菌株进行了军团菌毒力基因rcp的检测.结果 3株嗜肺军团菌扩增出了900 bp的rcp基因片段,其余菌株未扩增出该基因片段.结论 本研究建立的军团菌毒力基因rcp的PCR检测方法具有较高的特异性和敏感性,适于军团菌毒力基因的深入研究.“,”Objective To establish a polymerase chain reaction method for the detection of the rcp virulence gene of L. pneumophila. Methods Sixteen strains of Legionella pneumophila, L. dumiffii, L. bozemanii and L. Longbeachae isolated from the cooling towers of the centralized air-conditioning systems in Tianjin form 2005 to 2007 were detected by polymerase chain reaction method using L. pneumophila rcp virulence gene-specific primer according to GenBank published nucleotide sequence. Results The 900 bp rcp genetic fragment was amplified in three strains of L. pneumophila, while others not. Conclusion The rcp virulence gene-based polymerase chain reaction method for the detection of L. pneumophila has been successfully established and is the foundation for the studies of Legionella virulence.