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目的采用多重实时荧光PCR对一起疑似食物中毒的样本进行快速检测,结合病原学的结果,确定病原体。方法2015年5月广西东兰县发生了一起疑似细菌性食物中毒。应用多重实时荧光PCR方法对留存食品和患者肛拭子标本进行副溶血性弧菌4种毒力基因的检测,同时对样本进行病原学检测。结果共检测15份食品样本和12份肛拭子标本,除了从1份肛拭子标本中分离培养出O3∶K6副溶血性弧菌外,其余标本均未分离出食源性致病菌。从8份肛拭子标本中检出副溶血性弧菌毒力基因,其中所有菌株均携带tdh、tlh和orf8基因,3株携带trh基因。结论这是广西首次利用多重实时荧光PCR技术确诊由副溶血性弧菌引起的食物中毒。多重实时荧光PCR方法可以同时鉴定和检测副溶血性弧菌及其毒力基因,具有快速、灵敏、准确的优点,可为食源性暴发事件提供快速、可靠的检测结果。
Objective To detect multiple samples of suspected food poisoning by multiple real-time fluorescence PCR and to identify pathogens in combination with the results of etiology. Methods In May 2015, a suspected bacterial food poisoning occurred in Donglan County, Guangxi. Multiple real-time fluorescence PCR was used to detect the four virulence genes of Vibrio parahaemolyticus in the retained food and the patient’s anal swab specimens, and the etiology of the samples was tested. Results A total of 15 food samples and 12 samples of rectal swabs were detected. Except for the O3: K6 Vibrio parahaemolyticus isolated from one rectal swab, none of the samples were isolated from food-borne pathogens. Vibrio parahaemolyticus virulence genes were detected from 8 samples of anal swabs, all of which carried the tdh, tlh and orf8 genes and 3 of them carried the trh gene. Conclusion This is the first time that Guangxi has used multiplex real-time PCR to diagnose food poisoning caused by Vibrio parahaemolyticus. Multiplex real-time fluorescent PCR can simultaneously identify and detect Vibrio parahaemolyticus and its virulence genes, and has the advantages of fast, sensitive and accurate, and can provide fast and reliable detection results for foodborne outbreaks.