组织培养是生物学和医学科学研究的一项常用技术,经常需要对培养细胞和接种病毒的细胞以及病毒颗粒进行超微结构的观察。一般常规组织培养细胞包埋技术是从培养瓶上把培养细胞刮下来,在随后的标本制备中,由于细胞不成团,需要反复离心,细胞损失量大。因此,每制作一次标本,往往需几个培养瓶的细胞,这实际上是难以满足的,并且在反复离心中,温度不易保持恒定,这种温度变化,常在标本结构上造成很多人为损伤。所 Tissue culture is a commonly used technique in both biology and medical science and often requires ultrastructural observation of cultured and virus-inoculated cells as well as virions. Conventional Tissue Culture Cell Embedding Techniques are used to scrape cultured cells out of a culture flask. In subsequent specimen preparation, cells are centrifuged repeatedly due to non-lumping of cells, resulting in large cell loss. Therefore, every time a specimen is made, several bottle-shaped cells are often required. This is actually difficult to satisfy. In repeated centrifugation, the temperature is not easily kept constant. Such temperature changes often cause a lot of man-made damage to the specimen structure. The