In this manuscript,we will present two related studies on decoding human genome with 3D chromatin interaction data.In the first study,we systemically investigate the multivalent functions of CTCF from chromatin state and three-dimensional chromatin interactions.CCCTC-binding factor (CTCF) is a multi-functional protein that is assigned various,even contradictory roles in the genome.High-throughput sequencing-based technologies such as ChIP-seq and Hi-C provided us the opportunity to assess the multivalent functions of CTCF in the human genome.The location of CTCF-binding sites with respect to genomic features provides insights into the possible roles of this protein.Here we present the first genome-wide survey and characterization of three important functions of CTCF: enhancer insulator,chromatin barrier and enhancer linker.We developed a novel computational framework to discover the multivalent functions of CTCF based on chromatin state and three-dimensional chromatin architecture.We applied our method to five human cell lines and identified ~46000 non-redundant CTCF sites related to the three functions.Disparate effects of these functions on gene expression were found and distinct genomic features of these CTCF sites were characterized in GM12878 cells.Finally,we investigated the cell-type specificities of CTCF sites related to these functions across five cell types.Our study provides new insights into the multivalent functions of CTCF in the human genome.