为提高大豆的耐旱耐盐碱性,将拟南芥转录因子DREB2A序列优化后,构建了植物表达载体pCAMBIA3300-DREB2A,利用农杆菌介导法将DREB2A基因导入大豆品种Willimas 82和Bert中,转化率分别为0.8%和3.2%。对抗性转基因植株进行PCR检测,2个品种分别获得阳性植株28和63株,表明DREB2A已整合到大豆基因组中。 In order to improve the drought tolerance and salt-tolerance of soybean, the plant expression vector pCAMBIA3300-DREB2A was constructed after optimization of the Arabidopsis transcription factor DREB2A sequence. The DREB2A gene was introduced into Willimas 82 and Bert by Agrobacterium-mediated transformation and transformed Rates were 0.8% and 3.2% respectively. The resistant transgenic plants were tested by PCR, 28 and 63 positive plants were obtained from 2 cultivars, respectively, indicating that DREB2A has been integrated into soybean genome.