目的 :了解胆囊癌患者胆囊癌根治手术及复发转移后HIF-1α的变化特点和临床意义。方法 :选取我院2010年1月~2011年9月收治的胆囊癌患者,其中男36例,女52例,用酶联免疫吸附试验法检测患者手术前1天、术后1周和术后2周的血清HIF-1α水平,观察其动态变化,分别记录其复发转移情况,比较胆囊癌复发转移患者治疗后2周与复发转移时血清HIF-1α含量,通过血清HIF-1α含量判断胆囊癌患者手术治疗效果及预测预后的价值进行评价。结果 :术后1周较术前1天明显降低,且术后2周较术后1周稍有下降。血清HIF-1α水平增高与胆囊癌患者TNM分期Ⅰ~Ⅳ分级、肿瘤体积及肿瘤转移的阴阳性率密切相关,但两组的HIF-1α值在年龄(50岁为界限)及性别方面无明显差异。结论 :HIF-1α表达与胆囊癌病人疾病进展明显相关,检测患者血清HIF-1α的动态变化,可以作为评判患者病情的有效临床生化指标,灵敏度和准确度均较高,可为制定个体化手术方案提供依据,具有重要的临床应用价值。 Objective: To investigate the characteristics and clinical significance of the changes of HIF-1α in patients with gallbladder carcinoma after radical operation and recurrence and metastasis. Methods: A total of 36 males and 52 females with gallbladder cancer admitted to our hospital from January 2010 to September 2011 were enrolled. One day before surgery, one week after surgery and after operation 2 weeks of serum HIF-1α levels were observed dynamic changes, respectively, the recurrence and metastasis were recorded, the recurrence and metastasis of patients with gallbladder cancer recurrence and metastasis after 2 weeks of serum HIF-1α levels, serum HIF-1α levels to determine the gallbladder cancer The effect of surgical treatment and prognosis of patients were evaluated. Results: One week after operation was significantly lower than that of one day before operation, and decreased slightly after two weeks of operation compared with one week after operation. The level of serum HIF-1α was closely related to TNM stageⅠ-Ⅳ grade, tumor volume and the positive rate of tumor metastasis in patients with gallbladder cancer, but there was no significant difference between the two groups in age (50 years) and sex difference. Conclusion: The expression of HIF-1α is significantly correlated with the progression of gallbladder cancer. Detecting the dynamic changes of serum HIF-1α in patients with HIF-1α can be used as an effective clinical and biochemical index to judge the severity of the disease. The sensitivity and accuracy of HIF- Program provides the basis, has important clinical value.