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利用次黄嘌呤(HPX)与黄嘌呤氧化酶(XO)在体外产生自由基的方法,观察对培养神经细胞的损伤,井着重了解pH变化、缺糖和兴奋性氨基酸对自由基损伤的影响。 取Wistar大鼠15～16日胚胎的大脑皮层组织,
The in vitro production of free radicals by hypoxanthine (HPX) and xanthine oxidase (XO) was used to observe the damage to cultured nerve cells and to understand the effects of pH changes, glucose deprivation and excitatory amino acids on free radical damage. Wistar rats from 15 to 16 embryonic cerebral cortex tissue,