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目的 研究大鼠前脑内星形胶质细胞 (ASs)及神经元 (Ns)对一侧胫、腓骨骨折的反应及相互关系。方法 应用细胞免疫组织化学方法观察前脑内胶质原纤维酸性蛋白 (GFAP)、Fos蛋白以及酪氨酸羟化酶 (TH)在左侧胫、腓骨骨折后的表达变化。 结果 1 一侧胫、腓骨骨折后 ,前脑GFAP阳性表达的ASs有明显的核团定位。在缰外侧核内侧区 (LHb)、下丘脑室旁核 (Pa)、视上核 (SON)、视交叉上核 (SCh)、终纹床核 (BST)、杏仁中央核 (Ce)和内侧杏仁核 (Me)及皮层等脑区内均可观察到有GFAP阳性ASs分布 ,且两侧分布无明显差异。 2 Fos阳性Ns的分布与GFAP阳性ASs上述分布部位基本一致 ,两者关系密切。 3 Pa等部位有大量Fos TH双标Ns,四周是密集的GFAP阳性ASs包围 ,形成以神经元为中心 ,周围包绕ASs,共同构成神经元 星形胶质细胞复合体 (N ASC)。 结论 上述核团的ASs参与了下肢骨折伤害性刺激的应激反应及其调节过程 ,且与Ns关系密切 ,可能主动地影响Ns的活动。
Objective To investigate the response of astrocytes (ASs) and neurons (Ns) to the tibiofibular fractures of the rat’s anterior forebrain and their relationship. Methods The expression of glial fibrillary acidic protein (GFAP), Fos protein and tyrosine hydroxylase (TH) in the left forelimb of tibia and fibula was observed by immunohistochemistry. Results One side of the tibia and fibula fracture, the forebrain GFAP positive expression of ASs obvious nuclear localization. (LH), Pa, SON, SCh, BST, Ce, and medial Amygdala (Me) and cortex and other brain areas were observed GFAP positive ASs distribution, and no significant difference between the two sides of the distribution. 2 Fos-positive Ns distribution and GFAP-positive ASs basically the same distribution of the site, the two are closely related. 3 Pa and other parts of a large number of Fos TH double-labeled Ns, surrounded by dense GFAP positive ASs surrounded by the formation of neurons as the center, surrounded by ASs, together constitute the neuronal astrocyte complex (N ASC). Conclusion The ASs of the above nuclei are involved in the stress response and regulation process of lower extremity fracture noxious stimuli, and are closely related to Ns and may have a negative impact on the activity of Ns.