论文部分内容阅读
目的探讨PTEN蛋白在子宫内膜癌发生发展中的可能机制。方法收集子宫内膜癌50例、子宫内膜增生症25例和正常子宫内膜组织30例,采用免疫组化、Western blot方法检测子宫内膜中的PTEN蛋白表达,研究其与子宫内膜癌进展的相关性。结果与正常子宫内膜组织和子宫内膜增生症的子宫内膜相比,子宫内膜癌中的PTEN蛋白表达率明显降低(P=0.042,0.017),Ⅰ期、Ⅱ期、Ⅲ期子宫内膜癌的PTEN蛋白阳性表达率差异均无统计学意义(P>0.05),高分化、中分化、低分化的PTEN蛋白阳性表达率差异亦无统计学意义(P>0.05)。结论 PTEN蛋白在子宫内膜癌表达率降低,提示PTEN基因可能具有抑制子宫内膜过度增生及癌变发生的作用。
Objective To investigate the possible mechanism of PTEN protein in the development of endometrial carcinoma. Methods Fifty cases of endometrial carcinoma, 25 cases of endometrial hyperplasia and 30 cases of normal endometrium were collected. The expression of PTEN protein in endometrium was detected by immunohistochemistry and Western blot, and the relationship between endometrial carcinoma and endometrial carcinoma Progress relatedness. Results Compared with normal endometrium and endometrial hyperplasia, the expression of PTEN protein in endometrial carcinoma was significantly lower (P = 0.042,0.017), stage I, II and III uterus There was no significant difference in the positive expression rates of PTEN protein between the two groups (P> 0.05). There was no significant difference in the positive expression rate of PTEN protein between the well-differentiated, moderately differentiated and poorly differentiated tissues (P> 0.05). Conclusions The decreased expression of PTEN protein in endometrial carcinoma suggests that PTEN gene may have the effect of inhibiting endometrial hyperplasia and carcinogenesis.