ENDONUCLEASE相关论文
55. Mutagenic Analysis on the Polyhedrin gene (polh) of Bombyx mori Nuclear Polyhedrosis virus (BmNP
In our early studies, the abnormal shape of the polyhedra of Bombyx mori nuclear polyhedrosis virus (BmNPV) induced by c......
研究表明，肝脏缺血再灌注损伤(HIRI)与细胞凋亡密切相关。因此，实现细胞凋亡过程中生物标志物变化的监测，对于筛选新型肝脏缺血再灌注......
The"Switching-On" complexes,indolium derivatives bound with nuclear acids,were used as fluorescence probes to recogn......
C-erbB-2(HER-2 / neu) proto-oncogene is mainly expressed in epithelial tissue and activated due to its amplification. Am......
Sensitive detection of DNA methyltransferase activity based on rolling circle amplification technolo
This work develops a fluorescence approach for sensitive detection of DNA methyltransferase activity based on endonuclea......
Objectives The cellular repressor of El A-activated genes (CREG), a novel gene, was recently found to play a role in inh......
AIM:To establish a mice model harboring hepatitis B virusx gene(adr subtype)for studying the function of hepatitis Bvir......
Applying a highly specific and reproducible cDNA RDA method to clone garlic up-regulated genes in hu
AIM:To develop and optimize cDNA representationaldifference analysis(cDNA RDA)method and to identify andclone garlic up......
Secondary xylem development has long been recognized as a typical case of programmed cell death (PCD) in plants. During ......
AIM:To identify the gene that may predispose to humangastric cancer and to analyze its expression in gastric cancerand ......
Association of single nucleotide polymorphism of vitamin D receptor gene start codon and the suscepb
Aim: To investigate the single nucleotide polymorphism of vitamin D receptor (VDR) gene start codon in the Han nationali......
背景与目的存活素特异地在肿瘤组织中过表达,许多报道表明抑制它的功能对肿瘤治疗有利。RNA干扰被证明是一项能有效而特异地抑制基......
Objective: To express human telomeric repeat binding factor (TRF1) at high level in E. coli. Method: Two primers were d......
The peaT1 gene fragment was amplified from pGEM-6p-1-peaT1 by PCR,and recovered target gene was cloned into pLexA vector......
INTRODUCTIONEndogenesis infection plays an important role innosocomial infection.By studying progress ofbacteria transl......
Polymorphisms of interleukin-1B and interleukin-1 receptor antagonist genes in patients with chronic
AIM:To investigate the relationships between polymorphismsof interleukin-1B(IL-1B)promoter region -511C/T andinterleuki......
Tea polyphenols increase X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonu
Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprot......
Restriction fragments of HBV-DNA, cleaved by endonuclease HhaI,containing HBcAg gene were trimmed by BAL-31 exonuclease ......
Human mitochondrial DNA (mtDNA) restriction endonuclease fragment patterns were analyzed using placenta DNA isolated fr......
The entire coat protein (CP) gene and part of the 3’-noncoding sequence of the potatovirus Y (PVY, the Chinese isolate)......
In the search for the genome of egg drop syndrome virus (EDSV-76) Chinese strain AAV-2, part of restrietion endonucleas......
THE EUKARYOTIC EXPRESSION OF HUMAN PERFORIN PROTEIN AND THE ESTABLISHMENT OF HYBRIDOMAS PRODUCING AN
Objective To prepare the monoclonal antibody against human perforin(HP). Methods Recombi- nant eukaryotic expression pla......
AIM:To construct a recombinant E.coli strain that wouldhighly express the proinflammatory outer membraneprotein of huma......
Pilot study on the mechanism and effect of curcumin on oxidative stress and insulin secretion in INS
Objective To observe the effect of curcumin on the oxidative stress and glucose-stimulated insulin secretion(GSIS) and t......
Pathogenicity of diatraea saccharalis Densovirus to Host Insets and Characterization of its Viral Ge
Pathogenicity of the Diatraea saccharalis densovirus (DsDNV) was tested on its host larvae. The results showed that up t......
The influenza virus RNA-dependent RNA polymerase is a heterotrimeric complex (PA, PB1 and PB2) with multiple enzymatic a......
Objective To investigate the possible mechanism of endonuclease G(Endo G)-mediated non-Gaspase-dependent apoptotic pathw......
Objective:To construct and identify recombinant plasmid pUIS3-BLC~+.Methods:The cDNA of B-lymphocyte chemoattractant(BLC......
Construction and Identification of a Yeast Two-Hybrid Bait Vector and Its Effect on the Growth of Ye
By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HP......
Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expressio
AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion......
Rad51/RadA paralogs found in eukaryotes and euryarchaea play important roles during recombination and repair,and mutatio......
Expression of angiostatin cDNA in human gallbladder carcinoma cell line GBC-SD and its effect on end
AIM: To explore the influence of angiostatin up-regulation on the biologic behavior of gallbladder carcinoma cells in vi......
Targeted genome editing technology has been widely used in biomedical studies. The CRISPRassociated RNA-guided endonucle......
Objective: To disclose the nature of RBI germline mutations in Chinese and to develop a practical and effective way for......
Purpose: To construct the enhanced yellow fluorescent protein (EYFP) vector carryinginterferon-γ gene (ifn-γ) in order......
Objective:To construct siRNA recombinant expression vector targeting Livin gene and observe the apoptosis induction effe......
Enhanced Chemotherapy Sensitivity of Human Colon Cancer Cells to 5-Fluorouracil by siRNA Recombinant
Objective To investigate the effects of small interfering RNA(siRNA) recombinant expression vector targeting survivin ge......
Objective:We recombine the suicide gene CD,UPRT into plasmid pTRKH2 and clone the recombinant dual suicide gene therapy ......
Objective:To ascertain the variations of mitochondrion DNA(mtDNA) in mouse tumors and to inquire into the relationship b......
AIM: To investigate a therapeutic method for gastrointestinal stromal tumor (GIST) based on KIT RNA interference (RNAi) ......
The HsaI restriction enzyme from the embryos of human, Homo sapiens, has been isolated with both the tissue extract and......
Utilizing restriction endonuclease mapping and blot bybridization, we have determined the arrangement of the α-globin ......
Hepatitis B virus surface antigen (HBsAg) inRNA has been enriched from a hepatoma cell line (PLC/PRF/5) by specific pol......
SITE-SPECIFIC DELETION OF THE N-TERMINAL SEGMENTS FROM EcoRI ENDONUCLEASE USING THE POLYMERASE CHAIN
We developed a general and efficient method for directing the deletions of DNA sequences of any lengths using polymerase......
目的 牛分枝杆菌存在pncA基因C169G和oxyR基因G285A突变,而结核分枝杆菌则无此突变,本研究利用DHPLC和SURVEYOR酶法测定这两个位点......
Effect of EME1 exon variant Ile350Thr on risk and early onset of breast cancer in southern Chinese w
Essential meiotic endonuclease 1 homolog 1 (EME1) is a key DNA repair protein that participates in the recognition and r......
