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牛脑充分匀浆后经三次硫酸铵分级沉淀,再通过一次DEAE-Sepharose CL-6B层析柱,线性梯度洗脱后共收集4个峰洗脱液。PAGE分析(7.5％凝......
1,4-Dihydropyridine and polyhydroquinoline derivatives have been prepared efficiently in a one-pot synthesis via Hantzsc......
The strong yellow upconversion (UC) light emission was observed in Ho3+/Yb3+ co-doped Gd2Mo3O9 phosphor under the excita......
Morschauser(1988)has argued, in this writer＇s view convincingly,thatsdf3-tryt is not to be understood as an oath of alleg......
Plasma membrane (PM) proteome is one of the major subproteomes present in the cell,and is very important in liver functi......
Determination of the Content of Total Saponins in Paris daliensis H.Li et V. G. Soukoup and Paris du
[Objectives]To determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H......
Inhibition of Hepatitis B Virus Replication by Rheum palmatum L. Ethanol Extract in a Stable HBV-pro
Hepatitis B virus(HBV) infection is a severe health problem in the world. However, there is still not a satisfactory the......
Polyamidoamine (PAMAM) dendrimers are new artificial macromolecules with tree-like structure and have characteristics of......
Inoculation with purified specific protein is usually the first step for preparation of monoclonal antibody (mAb).But i......
Enhancement of binding activity of soluble human CD40 to CD40 ligand through incorporation of an iso
Aim:To investigate the effect of incorporation of an isoleucine zipper(IZ)motifinto CD40 on binding activity of CD40 fo......
AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by R......
Cloning,expression,purification and bioinformatic analysis of 2-methylcitrate synthase from Mycobact
Objective:To clone,express and purify 2—methylcitrale synthase(Rv1131) gene of Mycobacterium tuberculosis(M.tuberculosi......
A superoxide dismutase(SOD) was purified to homogeneity from fresh camellia pollen by means of ammonium sulfate precipit......
Isoentropic and Isoenthalpic Temperatures of Protein Unfolding in Hydrophobic Interaction Chromatogr
The thermal behaviors of five proteins in hydrophobic interaction chromatography (HIC) were investigated in the temperat......
Synthesis of Fine Y_2O_3:Eu~(3+) Phosphor by Solid-State Reaction at Ambient Temperature and Its Lum
Precursor was synthesized by solid-state reaction at room temperature among Y(NO3)3·6H2O, Eu2O3 and C2H2O4·2H2O. Y2O3......
SYNTHESIS OF FUNCTIONAL MACROMOLECULE INTERMEDIATE THROUGH ACYLATION CATALYZED BY [Emim]Cl-AlCl_3 IO
Acylation reaction of anthracene with oxalyl chloride in the presence of [Emim]C1-A1C13 ionic liquid has been investigat......
ZnS nanoparticles were prepared with normal ZnO and Na2S by solid-liquid chemical reaction under ultrasonic condition an......
Mechanistic Aspects of Monomer, Polymer Formation, and Synthesis of PQ-Alt-Dialkyl-fluorene Conjugat
Suzuki coupling reaction is widely used in the construction of conjugated polymers; however, there is still no report de......
Amphibian skin contains rich bioactive peptides. Especially, a large amount of antimicrobial peptides have been identifi......
Poly(styrene-co-sodium styrene sulphonate)(P(St-NaSS)) latex particles were prepared using the mixture of ethanol and wa......
目的:对大孔树脂富集纯化曲札芪苷的工艺条件进行优化.方法:采用静态及动态吸附-解吸试验方法,以曲札芪苷的含量为指标进行考察.结......
本研究以吸附率、解吸率为考察指标,采用静态吸附方法筛选出纯化效果最佳的LSA-21型树脂。考察各种因素对树脂吸附、解吸效果的影......
利用大肠杆菌表达表皮生长因子受体胞外段(ED-EGFR),将目的基因片段插入到pET28a载体中,将插入目的基因载体转化入BL21表达.利用异......
,Purification and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase from European Pilchar
The NAD+-dependent cytosolic glyceralehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) was purified from the skeletal......
WRKY proteins are transcriptional regulators involved in plant responses to biotic and abiotic stresses, metabolisms, an......
Effect of Polysaccharides from Fruit Body of Ganoderma tsugae on Bidirectional Regulation of Proinfl
The polysaccharides,FIII-2,FIII-2-a,FIII-2-b and FIII-2-c were purified from the fruit body of Ganoderma tsugae(G.tsugae......
Extraction behaviours of titanium and other impurities in the decomposition process of ilmenite by h
To effectively utilize ilmenite and recycle KOH solution, the extraction behaviours of titanium and other associated imp......
目的：建立一种简单、稳定、高效的新生大鼠背根神经节神经元原代培养方法。方法：摘取新生24h SD大鼠背根神经节，采用0.25%胰酶和0.1%......
目的对吐鲁番葡萄树伤流液多糖进行分离纯化,并分析其单糖组成。方法采用醇沉法提取多糖,Sevage法脱蛋白,经DEAE-52纤维素柱及Seph......
报道重组点状产气单胞菌脯氨酰内肽酶 (简称apPEP)的基因工程下游工艺研究。工程菌株E .coliBL2 1/ pKKH PEP表达产物apPEP为可溶......
目的通过大孔吸附树脂富集纯化玳玳花总黄酮。方法静态吸附实验考察NKA-9、HPD100、HPD400、HPD600、AB-8、D101、X-5、DM301大孔......
目的克隆甘油脱水酶α亚基基因，构建表达载体，表达并纯化融合蛋白。方法采用PCR技术克隆甘油脱水酶α亚基gldA基因，并将其重组到含组......
从土壤中分离出1株可产抗真菌蛋白的菌株,通过形态观察和分子生物学实验等方法鉴定其为巨大曲霉,编号AF-11。研究证明,利用纤维素......
原核表达获得的重组牛凝乳酶与商品重组牛凝乳酶有相似的酶学特性，具商业潜力，故进一步研究其纯化方法。使用离心与饱和硫酸铵沉淀，成......
目的苯酚抽提法和脱氧胆酸钠沉淀法去除14型肺炎链球菌荚膜多糖中蛋白质的效果比较。方法将3批次14型肺炎链球菌发酵培养液经超滤......
以鲫鱼肌肉为原料提取乙酰胆碱酯酶（Acetylcholinesterase,AChE）,然后采用改进的Ellman方法按照正交试验设计分别测定不同环境条件（pH......
概述了SSR标记在棉花（Gossypium spp.）的指纹图谱构建、遗传多样性分析、品种纯度检测、品种间差异性相关分析等方面的研究进展,并对......
研究了嘉祥白菊花中黄酮类化合物的提取分离和结构鉴定。采用超声波提取法进行提取总黄酮，制得黄酮浸膏。样品经大孔树脂柱、硅胶柱......
通过单因素实验和均匀设计实验对一株产果糖基转移酶的米曲霉摇瓶发酵条件进行优化,其最佳发酵条件为：发酵温度24℃,转速161r/min,......
本文以三萜类化合物的洗脱效果为考察指标,研究适合芡实壳三萜类化合物分离纯化的大孔树脂吸附性能及洗脱参数。研究结果显示DM301......
通过比较D101、AB-8、HPD400、HPD500、HPD417、HPD826六种大孔树脂的静态吸附效果，从中筛选出适合分离新疆圆柏总黄酮的树脂，并在单......
利用兰州SFC加速的16O束轰击同位素118Sn,由熔合蒸发4n反应产生目标核130Ce.为了消除本底干扰并指定130Ce核,采用溶剂萃取法对He-j......
分析了燃用重油造成滑油污染的相关因素、特征及后果。介绍了燃重油柴油机对滑油特性指标要求。针对滑油净化处理装置的使用现状，提......
用ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０和ＳｅｐｈａｄｅｘＧ－７５柱层析，从五步蛇粗毒中分离纯化出一种促凝组分（类凝血酶），并对其纯度和促凝活性作鉴定。......
目的优选金樱叶总皂苷的提取、纯化工艺条件。方法以齐墩果酸含量为指标,采用L9（34）正交试验设计,优选金樱叶提取工艺条件;以金樱叶......
生长素结合蛋白能够与生长素特异性结合，因而有可能直接被用作生长素免疫分析和生物传感测定中的高特异性、高亲和力识别分子。本研......
研究利用黑曲霉发酵强化对草石蚕的提取,运用高效液相色谱-示差折光检测法（HPLC-RID）对得到的水苏糖进行分析。采用单因素实验方法,......
利用来源于蓝细菌Synechocystis sp．PCC6803的Dna B内舍肽的剪切特性，纯化绿色荧光蛋白．在大肠杆菌中将内含肽与绿色荧光蛋白融合表达......
为制备小熊猫白介素17（IL-17）表达产物，提取活化的小熊猫外周血单个核细胞总 RNA，合成cDNA，以此为模板克隆 IL-17的成熟肽编码序列，将其......
