【摘 要】
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Objective To investigate the signaling pathways involved inKAI1-reduced vascular endothelial growth factor C (VEGF-C) down-regulation and lymphatic metastasis in MIA PaCa-2 pancreatic cancer cells.Mat
【机 构】
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Department of Gastroenterology, General Hospital of Shenyang Military Area
【出 处】
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The 14th Congress of Gastroenterology China(第十四届全国消化系病学术会议)
论文部分内容阅读
Objective To investigate the signaling pathways involved inKAI1-reduced vascular endothelial growth factor C (VEGF-C) down-regulation and lymphatic metastasis in MIA PaCa-2 pancreatic cancer cells.Material and Methods MIA PaCa-2 pancreatic cancer cells were transfected with KM1 by liposomes.The expression level of VEGF-C was assessed by Western blot.Levels of vascular endothelial growth factor (VEGF)-C secreted by cells were measured by enzyme-linked immunosorbent assay (ELISA).Src and STAT3 phosphorylation was detected by Western blot.Signaling transduction inhibitors, PP2 and AG490, were used to block Src and STAT3 signaling pathways, respectively.
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