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目的为研究铝毒及耐铝机制提供更好的模式生物材料和进一步研究耐铝机制提供依据。方法通过对云南龙陵县茶园土壤耐酸铝微生物的筛选、分离和纯化,筛选出一株Y31耐酸铝酵母。结果经D1/D2区域克隆测序获取全长26S rDNA区域序列,构建系统发育树,初步鉴定Y31为长形隐球酵母(Cryptococcus longus)。在LPM培养基及GM培养基上耐铝水平检测表明Y31分别能耐100 mmol/L和50 mmol/L铝。用不同铝浓度处理酵母菌后,进行FDA PI双染色并用荧光显微镜观察,当铝浓度达到100 mmol/L时,出现明显的细胞凋亡过程。结论本研究为铝毒和生物体耐铝机制提供了生物材料。
OBJECTIVE: To provide a basis for the study of aluminum toxicity and aluminum tolerance mechanism to provide better model biomaterials and to further study the mechanism of aluminum tolerance. Methods Screening, isolating and purifying the aluminum-tolerant aluminum-bearing microorganisms in the tea garden of Longling County, Yunnan Province, China, screened a Y31 acid-resistant aluminum yeast. Results The full-length 26S rDNA sequence was obtained by cloning and sequencing of D1 / D2 region, and phylogenetic tree was constructed. The preliminary identification of Y31 was Cryptococcus longus. Aluminum tolerance test on LPM medium and GM medium showed that Y31 could tolerate 100 mmol / L and 50 mmol / L aluminum, respectively. After treatment of yeast with different concentrations of aluminum, FDA PI double staining and observation with a fluorescence microscope showed obvious apoptosis process when the aluminum concentration reached 100 mmol / L. Conclusions This study provides biomaterials for mechanisms of aluminum toxicity and tolerance to aluminum in organisms.