论文部分内容阅读
目的:探讨单次癫痫发作后脑内NF-κB和N-Cadherin表达与海马结构中苔藓纤维发芽现象之间的关系与作用。方法:利用腹腔注射戊四氮(PTZ)制作发育期SD大鼠(14 d、28 d)单次惊厥发作模型,各日龄组随机分为生理盐水(NS)组、PTZ组、吡咯二硫氨基甲酸酯(PDTC)组和PDTC+PTZ组,采用免疫组化法检测NF-κB和N-Cadherin的表达,利用Timm染色法观察海马苔藓纤维发芽现象。结果:NS组在海马CA3区可见极少Timm染色颗粒;致惊后1周偶见Timm染色颗粒、3周可见Timm染色颗粒沿海马CA3区呈条带样分布(P<0.01);PDTC预处理组Timm染色颗粒较PTZ致惊组明显减少(P<0.05)。NS组大鼠海马CA1、CA3区无NF-κB p65核转位细胞,致惊后24 h各日龄组幼鼠海马CA1、CA3区NF-κB p65核转位细胞较NS组明显增加(P<0.01);PDTC预处理后NF-κB p65的核转位细胞较致惊组明显减少(P<0.05)。NS组海马CA1、CA3区可见少量N-Cadherin阳性细胞;致惊组海马CA3和齿状回门区的N-Cadherin的阳性细胞与NS组相比明显增多(P<0.01),PDTC预处理后相同区域内N-Cadherin阳性细胞较PTZ致惊组明显减少(P<0.05)。NF-κB p65、N-Cadherin及Timm染色颗粒的表达结果与惊厥鼠的日龄并无关联性。结论:幼鼠单次惊厥发作可以引起海马不同程度的苔藓纤维发芽,而NF-κB p65、N-Cadherin的分布位置与变化时相与苔藓纤维发芽相吻合,表明NF-κB p65、N-Cadherin可能参与或伴随着苔藓纤维的发芽。
Objective: To investigate the relationship between the expression of NF-κB and N-Cadherin in brain and the mossy fiber germination in hippocampal formation after single epileptic seizure. Methods: The single seizure model was established by intraperitoneal injection of pentylenetetrazol (PTZ) in developing SD rats (14 d, 28 d). The rats of each age group were randomly divided into NS group, PTZ group, Carbamate (PDTC) group and PDTC + PTZ group. The expressions of NF-κB and N-Cadherin were detected by immunohistochemistry and the germination of mossy fiber in hippocampus was observed by Timm staining. RESULTS: Timm-stained granules were found rarely in CA3 area of hippocampus in NS group. Timm-stained granules were occasionally seen at 1 week after stunned. Timm-stained granules showed a band-like distribution in CA3 area of hippocampus at 3 weeks (P <0.01) Compared with PTZ-induced group, the Timm-stained particles in the group decreased significantly (P <0.05). In NS group, there was no nuclear translocation of NF-κB p65 in the hippocampal CA1 and CA3 regions. The expression of NF-κB p65 nuclear translocating cells in hippocampal CA1 and CA3 of the 24-hour-old rats increased significantly compared with the NS group (P <0.01). The number of NF-κB p65 nuclear translocation cells in PDTC group was significantly decreased after PDTC pretreatment (P <0.05). A small amount of N-Cadherin positive cells were observed in hippocampal CA1 and CA3 area of NS group. N-Cadherin positive cells in hippocampal CA3 and dentate gyrus in hippocampus group were significantly increased compared with NS group (P <0.01) N-Cadherin-positive cells in the same area were significantly lower than those in PTZ-induced group (P <0.05). The expression of NF-κB p65, N-Cadherin and Timm staining particles had no correlation with the age of the convulsive mice. CONCLUSION: Single seizures in young rats can induce different levels of mossy fiber germination in the hippocampus. The distribution and location of NF-κB p65 and N-Cadherin coincide with mossy fiber germination, indicating that NF-κB p65 and N-Cadherin May be involved in or accompanied by sprouting of mossy fibers.