目的　对我国血清学调查中发现的四型 (G型 )轮状病毒NSP4序列进行测定和分析 ,了解NSP4的基因类型和变异状况。方法　利用银染测序方法对G1、G2、G3、G9型毒株NSP4cDNA序列进行测定。结果　四个血清型毒株NSP4的核苷酸和氨基酸序列的同源性分别为 78 8% ,93 5 % ;83 0 % ,95 5 %。G1、G3、G9型标本多属Wa组 ;G2型标本多属KUN组。NSP4的变异主要位于VP4结合域内的 135 145位氨基酸。结论　Wa组和KUN组是我国主要的两种NSP4基因型。 Objective To detect and analyze the type Ⅳ (G) rotavirus NSP4 sequence found in the serological investigation of our country to understand the gene type and variation of NSP4 gene. Methods The sequence of NSP4 cDNA of G1, G2, G3 and G9 strains was determined by silver staining. Results The nucleotide and amino acid sequences of four serotypes of NSP4 were 78 8%, 93 5%, 83 0% and 95 5%, respectively. G1, G3, G9 specimens are mostly Wa group; G2 specimens are mostly KUN group. The variation of NSP4 is mainly located at amino acid 135 145 in the VP4 binding domain. Conclusion Wa and KUN groups are the two major NSP4 genotypes in China.