论文部分内容阅读
目的 :研究汉族国人中 β2 肾上腺素受体( β2 AR )遗传多态性与夜间哮喘的相关性。方法 :应用聚合酶链反应———等位基因特异性寡核苷酸杂交法 (PCR ASO)对夜间哮喘 2 5例 (NA组 )和非夜间哮喘 2 2例 (NNA组 )以及 72例健康人 (对照组 )的 β2 AR16和 2 7位的遗传多态性进行分析。所有受检者均为汉族国人。结果 :β2 AR 16位点Arg/Arg、Arg/Gly和Gly/Gly的基因型分布频率在NA组分别为 12 % ,16%和 72 % ;而在NNA组分别为 2 7%、41%和 3 2 %。与NNA组相比 ,NA组的基因型Gly/Gly频率和等位基因Gly频率明显增加 (P <0 0 1)。然而 ,NNA组与对照组的基因型Gly/Gly频率和等位基因Gly频率无显著差异 (P >0 0 5 )。β2 AR 2 7位点的基因型和等位基因型频率在三组间无显著差异 (P >0 0 5 )。结论 :β2 肾上腺素受体的Gly16多态性在夜间哮喘汉族国人中表达增强 ,与夜间哮喘具有相关性 ,可能为夜间哮喘这一表型的重要遗传因素
Objective: To investigate the association between β2 adrenergic receptor (β2 AR) genetic polymorphism and nighttime asthma in Han Chinese. Methods: Polymerase chain reaction-allele-specific oligonucleotide hybridization (PCR ASO) was used to evaluate the effects of nocturnal asthma in 25 cases (NA group) and non-nocturnal asthma in 22 cases (NNA group) The human (control group) β2 AR16 and 27 genetic polymorphisms were analyzed. All subjects were Han Chinese. RESULTS: The frequencies of genotypes Arg / Arg, Arg / Gly and Gly / Gly in the 12 AR2 sites were 12%, 16% and 72% in the NA group, while 27% and 41% in the NNA group 3 2%. Compared with NNA group, genotype Gly / Gly frequency and allele Gly frequency in NA group increased significantly (P <0.01). However, there was no significant difference in genotype Gly / Gly frequency and allele Gly frequency between NNA group and control group (P> 0.05). The genotype and allele frequencies of β2 AR 2 7 sites were not significantly different between the three groups (P> 0.05). CONCLUSION: The Gly16 polymorphism of β2-adrenergic receptor is enhanced in nocturnal asthmatic Han nationality people, which may be related to nocturnal asthma and may be an important genetic factor of nocturnal asthma phenotype