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目的 :了解HLC近交系小鼠骨髓微核自发率。方法 :将HLC小鼠、无毛小鼠及昆明种小鼠各分为 2组 ,一组取骨髓涂片观察 ,另一组腹腔注射 1 0 0mg/kg环磷酰胺 ,1 8h后取骨髓涂片观察 ,计算微核率。结果 :HLC近交系小鼠嗜多染红细胞微核自发率与无毛小鼠和昆明小鼠差异无统计学意义 (P >0 .0 5) ;腹腔注射环磷酰胺后 ,HLC近交系小鼠与无毛小鼠及昆明小鼠骨髓微核率均显著升高 (P <0 .0 1 ) ,但 3者之间差异无统计学意义 (P >0 .0 5)。结论 :HLC近交系与无毛小鼠及昆明小鼠微核自发率相似 ,对环磷酰胺诱变剂的敏感性相一致
Objective: To understand the spontaneous bone marrow micronucleus in HLC inbred mice. Methods: HLC mice, hairless mice and Kunming mice were divided into two groups. One group received bone marrow smears and the other group received intraperitoneal injection of cyclophosphamide at a dose of 100 mg / kg. Film observation, calculation of micronucleus rate. Results: There was no significant difference in spontaneous micronucleus rate between HLC inbred mice and hairless mice and Kunming mice (P> 0.05). After intraperitoneal injection of cyclophosphamide, HLC inbred lines The micronucleus rate of bone marrow of mice and hairless mice and Kunming mice were significantly increased (P <0.01), but there was no significant difference among the three groups (P> 0.05). CONCLUSIONS: The spontaneous rates of micronuclei in HLC inbred lines, hairless mice and Kunming mice are similar, and are consistent with the sensitivity of cyclophosphamide mutagens