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目的:探讨TLR4基因多态性与变应性鼻炎(AR)易感性的关系。方法:以212例AR患儿和169例健康对照儿童为研究对象,选取TLR4基因的rs10759930、rs2737190和rs2770150位点,以聚合酶链反应测序法完成基因型检测,以SPSS 17.0软件进行统计学分析,调查TLR4基因多态性位点分布特征,对AR和健康对照组儿童TLR4基因多态性位点的等位基因频率和基因型频率进行比较。结果:位点rs10759930的CT杂合(P=2.70×10~(-6),OR=2.66,95%CI1.76～4.00)及TT纯合突变(P=9.72×10~(-9),OR=8.78,95%CI 3.83～20.13)与AR具有相关性。结论:rs10759930的CT杂合及TT纯合突变均与AR的发病相关。
Objective: To investigate the relationship between TLR4 gene polymorphism and susceptibility to allergic rhinitis (AR). Methods: A total of 212 children with AR and 169 healthy children were enrolled in this study. The rs10759930, rs2737190 and rs2770150 loci of TLR4 gene were selected and genotypes were detected by polymerase chain reaction (PCR) sequencing. SPSS 17.0 software was used for statistical analysis To investigate the allele frequency and genotype frequency of TLR4 polymorphism in children with AR and healthy controls. Results: The heterozygote of CT (P = 2.70 × 10 -6, OR = 2.66, 95% CI 1.76-4.00) and TT homozygous mutation (P = 9.72 × 10 -9) in rs10759930, OR = 8.78, 95% CI 3.83 ~ 20.13) correlated with AR. Conclusion: The CT heterozygous and TT homozygous mutations of rs10759930 are both associated with the pathogenesis of AR.