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柑橘黄化脉明病毒(Citrus yellow vein clearing virus,CYVCV)和柑橘褪绿矮化病毒(Citrus chlorotic dwarf-associated virus,CCDaV)是2种最近在我国发生的柑橘病毒(Chen et al.,2014;Guo et al.,2015),主要通过带病苗木和昆虫传播(陈洪明等,2015),对我国柑橘产业造成了严重损失。目前尚无防治CYVCV和CCDaV的药剂,种植无病毒苗木和加强病害检测是重要的防治途径。当前虽已建立了CYVCV和CCDaV的RT-PCR检测方法(Loconsole et al.,2012;陈洪明等,2015),但鉴于田间可能存在
Citrus yellow vein clearing virus (CYVCV) and citrus chlorotic dwarf-associated virus (CCDaV) are the two most recently reported citrus viruses in our country (Chen et al., 2014; Guo et al., 2015), mainly through the transmission of diseased seedlings and insects (Chen Hongming et al., 2015), which caused serious losses to China’s citrus industry. There are currently no agents for controlling CYVCV and CCDaV. Planting virus-free seedlings and enhancing disease detection are important control measures. Although RT-PCR detection methods for CYVCV and CCDaV have been established (Loconsole et al., 2012; Chen et al., 2015), in view of the possible existence of the field