自Sanger测定胰岛素结构和Moore,Stein测定核糖核酸酶(RNA酶)结构以来,近二十年来蛋白质化学领域发展极为迅速,这些发展的一个重要方面是分析手段的超微量化,使蛋白质化学结构的研究进入一个新阶段。在各种超微量分析技术中,荧光法尤其引人注目。Udenfriend首先引入荧光胺试剂,将氨基酸、肽、蛋白质检测灵敏度提高到微微 Since the determination of insulin structure by Sanger and Moore, Stein determination of ribonuclease (RNase) structure, the rapid development of protein chemistry in the past two decades, an important aspect of these developments is the analytical means of ultra-micro-quantification of protein chemical structure Research into a new phase. In various ultra-trace analysis techniques, the fluorescence method is particularly attractive. Udenfriend first introduced fluorescent amine reagent, the amino acid, peptide, protein detection sensitivity increased to a slight