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甜菜未授粉胚珠接种在附加不同生长素(IAA、2,4—D)、细胞分裂素(KT、6BA)或激素组合(6BA+NAA和IAA+KT)的MS、PGOB、1/2MS等培养基上,可成功诱导出胚状体,分化诱导形成单倍体植株,对其用0.1％～03％秋水仙碱溶液处理使染色体加倍,获得了高度纯合的二倍体株系。试验选配了110种诱导培养基、两种分化和3种生根培养基,选择了143种不同基因型材料.不同基因型材料在不同培养基上的诱导率各不相同.
The non-pollination ovules of beet were inoculated with MS, PGOB, 1 / 2MS, etc. with different auxin (IAA, 2,4-D), cytokinin (KT, 6BA) or hormonal combination (6BA + NAA and IAA + KT) On the basis of this, we successfully induced the embryoid body and induced the formation of haploid plants. The chromosomes were doubled after being treated with 0.1% ~ 03% colchicine solution, and a highly homozygous diploid strain was obtained. In the experiment, 110 kinds of induction medium, two kinds of differentiation medium and three kinds of rooting medium were selected and 143 kinds of different genotype materials were selected.The induction rates of different genotypes on different medium were different.