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应用引物特异ＰＣＲ法对沈阳地区８３例各型丙型肝炎，６例原发性肝癌抗－ＨＣＶ及ＨＣＶＲＮＡ均阳性的血清标本进行ＨＣＶ基因分型，旨在了解本地区ＨＣＶ基因型分布情况及ＨＣＶ不同基因型与丙型肝炎病人临床病情轻重的关系。结果表明：ＨＣＶ－Ⅱ型感染占５７．３％（５１／８９）、ＨＣＶ－Ⅲ型感染占２８．１％（２５／８９）、Ⅱ／Ⅲ混合型感染占１３．５％（１２／８９）、未分型占１．１％（１８９）；急性肝炎、慢性肝炎轻度、中度、重度、肝炎后肝硬化及原发性肝癌中ＨＣＶ－Ⅱ型感染分别占６２．５％、３５．５％、６６．７％、８５．７％、９０．９％及１００％，而ＨＣＶ－Ⅲ型感染分别占２５％、４６．７％、８．３％、０％、９．１％及０％。提示沈阳地区ＨＣＶ感染以Ⅱ型为主，其次为Ⅲ型及Ⅱ／Ⅲ混合型，ＨＣＶ不同基因型与丙型肝炎病人临床病情的严重程度呈密切相关
The genotypes of HCV in 83 serotypes of hepatitis C and 6 cases of primary hepatocarcinoma in Shenyang were genotyped by primer-specific PCR to understand the distribution of HCV genotypes in this region and HCV The relationship between different genotypes and severity of hepatitis C patients. The results showed that HCV-Ⅱ infection accounted for 57.3% (51/89), HCV-Ⅲ infection accounted for 28.1% (25/89), and Ⅱ / Ⅲ mixed infection accounted for 13.5% (12/89 ), Sub-type accounted for 1.1% (189); acute hepatitis, chronic hepatitis, mild, moderate, severe, posthepatitic cirrhosis and primary liver cancer in HCV-type infection accounted for 62.5%, 35 .5%, 66.7%, 85.7%, 90.9% and 100% respectively, while HCV-Ⅲ infection accounted for 25%, 46.7%, 8.3%, 0% and 9.1% And 0%. It is suggested that type Ⅱ is the main type of HCV infection in Shenyang, followed by type Ⅲ and type Ⅱ / Ⅲ. The severity of HCV in HCV genotypes is closely related to the severity of HCV in patients with hepatitis C