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目的 :观察脑缺血再灌注过程中兴奋性氨基酸 (Excitatory amino acid,EAA)、氧自由基的变化 ,研究探索脑缺血再灌注损伤的机制。方法 :测定假手术组、缺血 30 min再灌注 60 min生理盐水 (NS)处理组和单唾液酸四已糖神经节苷脂 (GM1 ,1 0 mg/ kg,IP)处理组 ,鼠脑海马组织 EAA、丙二醛 (Malondiadehyde,MDA)的含量。实验应用全脑缺血 (4 VO)模型 ,EAA采用 HPLC法 ,MDA采用 TBA法。结果 :脑缺血再灌注 NS处理组海马组织 EAA含量明显较假手术组降低 (P<0 .0 1 ) ,MDA含量则显著性升高 (P<0 .0 1 ) ,GM1 处理组 EAA,MDA含量均无明显变化 (P>0 .0 5)。结论 :EAA、氧自由基参于脑缺血再灌注损伤机制
Objective: To observe the changes of excitatory amino acids (EAA) and oxygen free radicals during cerebral ischemia-reperfusion, and to explore the mechanism of cerebral ischemia-reperfusion injury. Methods: The sham-operation group, the NS group treated with 30 min reperfusion after ischemia for 60 min and the GM1, 10 mg / kg, Tissue EAA, malondiadehyde (MDA) content. The experimental model of global ischemia (4 VO), EAA using HPLC method, MDA using TBA method. Results: EAA content in hippocampus of rats in NS group was significantly lower than that in sham operation group (P <0.01) and MDA content was significantly increased (P <0.01) MDA content had no significant change (P> 0.05). Conclusion: EAA and oxygen free radicals participate in the mechanism of cerebral ischemia-reperfusion injury