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大豆灰斑病是由真菌类病原菌引发的,能导致大豆植物体整株死亡的恶性植物病害。hrpZpsta基因所编码的激发子harpin蛋白,已被证实能够通过引起植物过敏性反应来提高植株机体对多种病原菌的抗性。将含有hrpZpsta基因的植物表达载体转化到大豆品种吉农17和吉农29中,以所得的T4代株系为试验材料,通过分子生物学方法与人工接菌方式对其进行检测。Southern blot和RT-PCR鉴定结果表明hrpZpsta基因能够在吉农17和吉农29的T4代转化株系中稳定的遗传和表达,并且转化植物后代对灰斑病病原菌的抗性较受体品种明显提高。此外,不同大豆受体品种对灰斑病的抗性存在差异。
Soybean leaf spot disease is caused by fungal pathogens, can lead to the death of whole plant soybean plant pathogenic malignant plants. The harpin protein encoded by the hrpZpsta gene has been shown to increase plant resistance to various pathogenic bacteria by causing plant hypersensitivity reactions. The plant expression vector containing the hrpZpsta gene was transformed into the soybean varieties Jinong 17 and Jiunong 29, and the obtained T4 generation lines were used as materials for testing, and the molecular biology methods and artificial inoculation methods were used for testing the strains. The results of Southern blot and RT-PCR indicated that the hrpZpsta gene can be stably inherited and expressed in the T4 generation of Jinong 17 and Jienong 29, and the resistance of the progenies to gray spot pathogens was significantly higher than that of the recipient strains improve. In addition, there are differences in resistance to Cercospora zea between different soybean receptor varieties.