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为探讨非甾体类消炎药(NSAIDs)对幽门螺杆菌(HP)感染诱发的胃上皮细胞凋亡的影响,在功能性消化不良(FD)病人中,用抗CagA试验、原位末端标记、PCNA免疫染色等方法进行了研究。结果发现,凋亡率(AI)HP+或NSAIDs+且HP-组较HP-组明显升高,HP+组比NSAIDs+且HP+组高。增殖细胞阳性率(LI)HP+组高于HP-组和NSAIDs+且HP+组,在HP+组和NSAIDs+且HP+组中不论有无CagA表达AI或LI均无显著性差异。慢性胃炎组织学分级与凋亡细胞敷或PCNA标记细胞数间无相关性。提示NSAIDs能抑制HP引起的凋亡或增殖作用,且HP对胃上皮细胞增殖或凋亡的作用不受CagA表达及胃炎症程度的影响。
To investigate the effect of non-steroidal anti-inflammatory drugs (NSAIDs) on gastric epithelial cell apoptosis induced by Helicobacter pylori (HP) infection in patients with functional dyspepsia (FD), anti-CagA test, PCNA immunostaining and other methods were studied. The results showed that apoptosis rate (AI) HP + or NSAIDs + and HP- group was significantly higher than HP- group, HP + group than NSAIDs + and HP + group. The positive rate of proliferating cells (LI) in HP + group was higher than that in HP- group and NSAIDs + + HP + group. There was no significant difference in AI or LI between HP + + group and NSAID + + and HP + group with or without CagA expression. There was no correlation between histological grade of chronic gastritis and apoptotic cells or PCNA labeled cells. It is suggested that NSAIDs can inhibit the apoptosis or proliferation induced by HP, and the effect of HP on the proliferation and apoptosis of gastric epithelial cells is not affected by the expression of CagA and the degree of gastric inflammation.