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用导光棒经小鼠左下腹切口插入提睾肌囊内,切开阴囊暴露腹侧面提睾肌,在内透射照明下进行微循环观察,一次观察可连续2～3小时,微循环状态无明显改变。用NE、Ach局部滴加或尾静脉注射测试血管反应性,局部给药最小有效浓度NE为0.001ng/ml,Ach为0.01ng/ml;静脉给药最小有效量NE、Ach均为10ng。
The light guide rods were inserted into the cremaster muscle of the mice via the left lower abdomen incision and the scrotum was exposed to expose the cremaster muscle of the ventral surface. The microcirculation was observed under the internal transmission illumination. One observation could be continued for 2 ~ 3 hours. The microcirculation state Significant change. Vascular reactivity was tested by NE, Ach local injection or tail vein injection. The minimum effective concentration for local administration was 0.001 ng / ml for NE and 0.01 ng / ml for Ach. The minimum effective dose for intravenous administration of NE was 10 ng for Ach.