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测定和比较了远海梭子蟹(Portunus pelagicus)成熟卵巢和早期胚胎的水分、总脂、脂类组成和脂肪酸含量,结果表明:早期胚胎的水分含量显著大于成熟卵巢,但后者总脂/组织湿重含量显著小于前者,两者的总脂/组织干重并无显著差异;甘油三酰和磷脂含量在早期胚胎与成熟卵巢之间并不存在显著差异,但是早期胚胎中的游离脂肪酸和胆固醇含量均显著高于成熟卵巢,这可能是胚胎发育过程中脂类物质被水解成游离脂肪酸、胆固醇和甘油等物质;就脂肪酸组成而言,两者中均检测出27种已知脂肪酸,仅15∶0、14∶1n7、20∶1n7和18∶3n3 4种脂肪酸的含量存在显著差异(P<0.05),16∶0、18:0、16∶1n7、18∶1n9、20∶5n3和22∶6n3为成熟卵巢和早期胚胎中的主要的脂肪酸种类,其中n3-PUFA/n6-PUFA和20∶5n3/22∶6n3分别为3.5和0.7。
The moisture, total fat, lipid composition and fatty acid content of mature ovary and early embryo of Portunus pelagicus were determined and compared. The results showed that the moisture content of early embryo was significantly higher than that of mature ovary, The content of triglyceride and phospholipid did not show significant difference between early embryo and mature ovary, but both of the content of free fatty acid and cholesterol in early embryo Significantly higher than mature ovaries, which may be due to the hydrolysis of lipids into free fatty acids, cholesterol and glycerol during embryonic development. For fatty acid composition, 27 known fatty acids were detected in both, only 15: , 16: 1, 18: 0, 16: 1n7, 18: 1n9, 20: 5n3 and 22: 6n3 were significantly different (P <0.05) The major fatty acid species in mature ovaries and early embryos, with n3-PUFA / n6-PUFA and 20: 5n3 / 22: 6n3 being 3.5 and 0.7, respectively.