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目的:探讨吡格列酮对THP-1源性巨噬细胞ATP结合盒转运蛋白A1(ABCA1)、ATP结合盒转运蛋白G1(ABCG1)及B族Ⅰ型清道夫受体(SR-BⅠ)表达的影响及可能作用机制。方法:Real time PCR及Western-blot检测不同浓度吡格列酮(1,10,20,30μmol/L)作用24h,THP-1源性巨噬细胞ABCA1,ABCG1及SR-BⅠmRNA和蛋白的表达。使用过氧化物酶体增殖物激活型受体γ(PPARγ)特异性拮抗剂GW9662(2μmol/L)阻断吡格列酮(10μmol/L),24h后检测THP-1源性巨噬细胞ABCA1,ABCG1及SR-BⅠmRNA和蛋白的表达。结果:吡格列酮可浓度依赖性增高24hTHP-1源性巨噬细胞ABCA1,ABCG1及SR-BⅠmRNA和蛋白的表达,GW9662可显著抑制吡格列酮诱导的THP-1源性巨噬细胞ABCA1,ABCG1及SR-BⅠmRNA和蛋白表达的增高(P<0.05)。结论:吡格列酮激动PPARγ上调THP-1源性巨噬细胞胆固醇流出调节蛋白ABCA1,ABCG1及SR-BⅠ基因的表达。
OBJECTIVE: To investigate the effect of pioglitazone on the expression of ATP-binding cassette transport protein A1 (ABCA1), ATP binding cassette transport protein G1 (ABCG1) and type B scavenger receptor (SR-BⅠ) in THP- Possible mechanism of action. Methods: Real-time PCR and Western-blot were used to detect mRNA and protein expressions of ABCA1, ABCG1 and SR-BⅠ in THP-1-derived macrophages treated with different concentrations of pioglitazone (1,10,20 and 30μmol / L) Pioglitazone (10μmol / L) was blocked by GW9662 (2μmol / L), a specific antagonist of peroxisome proliferator-activated receptor γ (PPARγ), and ABCA1, ABCG1 SR-B I mRNA and protein expression. Results: Pioglitazone increased mRNA and protein expression of ABCA1, ABCG1 and SR-B Ⅰ in 24hTHP-1-derived macrophages in a concentration-dependent manner. GW9662 significantly inhibited pioglitazone-induced ABCA1, ABCG1 and SR-BImRNA And protein expression (P <0.05). CONCLUSION: Pioglitazone induces PPARγ up-regulation of cholesterol efflux modulators ABCA1, ABCG1 and SR-BⅠ in THP-1-derived macrophages.