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目的:探讨性染色体数目异常与男性肾透明细胞癌病理分级及临床分期之间的关系。方法:将20例男性肾透明细胞癌按照病理分级和临床分期分组,并选择5例正常肾组织作为阴性对照,用荧光原位杂交技术分析各例肿瘤细胞及对应癌旁细胞性染色体数目变异情况。以性染色体的获得和缺失为变异指标,分析其和肾癌病理分级及临床分期之间的关系。结果:肾癌细胞中性染色体获得显著高于癌旁及正常组织中的细胞(P<0.05);低分化组中肿瘤细胞性染色体获得高于高分化组(P<0.05);进展性肾癌较局灶性肾癌更易发生性染色体获得(P<0.05)。结论:男性肾透明细胞癌细胞性染色体的获得与肿瘤病理分级和临床分期有相关性。
Objective: To investigate the relationship between the number of abnormal sex chromosomes and the pathological grading and clinical stage of clear cell renal cell carcinoma in men. Methods: Twenty cases of renal clear cell carcinoma of the kidney were divided into three groups according to the pathological grade and clinical stage. Five normal renal tissues were selected as the negative control. Fluorescence in situ hybridization was used to analyze the variation of the number of chromosomes . With the acquisition and deletion of sex chromosome as the index of variation, the relationship between it and pathological grade and clinical stage of renal cell carcinoma was analyzed. Results: The number of metaphase chromosomes in renal cell carcinoma was significantly higher than that in para-cancerous and normal tissues (P <0.05). The tumor cells in poorly differentiated group were higher than those in well-differentiated group (P <0.05) Focal renal cell carcinoma was more likely to have sex chromosomes (P <0.05). Conclusion: There is a correlation between the acquisition of sex chromosomes in renal clear cell carcinoma and the pathological grading and clinical stage.