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The interaction of ruthenium(Ⅱ) polypyridyl complex with DNA has been studied by isothermal titrationcalorimetry (ITC). The results show that complex [Ru(phen)2PMIP]2+ {phen=1,10-phenanthroline, PMIP=2-(4-methylphenyl)imidazo[4,5-f]l,10-phenanthroline} in