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采用静水压休克方法研究了促使第二极体保留诱发三倍体水晶彩鲫的最佳条件。在卵受精后4—5min采用600kg/cm~2或650kg/cm~2的静水压处理3min,不但能导致100％的三倍化,而且胚胎的存活率相当高,孵化率为对照组的90％左右。研究表明,静水压休克是进行鱼类染色体组操作的有效方法,休克的最佳条件易于掌握,处理程序易于标准化。文中讨论了静水压处理的条件与三倍体出现率和胚胎存活率的关系,以及最佳条件下和不适条件下胚胎死亡的原因。
Hydrostatic shock method was used to study the second polar body to retain the best conditions induced by triploid crystal color crucian carp. Treating with hydrostatic pressure of 600kg / cm ~ 2 or 650kg / cm ~ 2 for 4-5min after egg fertilization can not only result in 100% triploidy, but also the survival rate of embryos is quite high. The hatching rate of control group About 90%. Studies have shown that hydrostatic shock is an effective method of fish genome manipulation, the best conditions for shock is easy to grasp, and the handling procedures are easy to standardize. The relationship of hydrostatic pressure to triploidy and embryo survival is discussed in this paper, and the reasons for embryo death under optimal conditions and conditions.