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目的:探讨散发性胆囊癌组织中错配修复基因hMLH1启动子甲基化状态和蛋白表达检测的临床意义。方法:收集我院胆囊癌患者47例,采用甲基化PCR和Envision免疫组化法检测其胆囊癌组织中hMLH1启动子甲基化状态和蛋白表达。结果:hMLH1启动子甲基化阳性率在肝脏浸润组间、淋巴结转移组间、Nevin分期之间差异均具有显著统计学意义(P<0.01或P<0.05)。hMLH1蛋白表达在hMLH1基因启动子组间、肝脏浸润组间、淋巴结转移组间、Nevin分期之间差异均具有显著统计学意义(P<0.01或P<0.05)。结论:散发性胆囊癌中启动子甲基化是hMLH1基因失活的原因之一,hMLH1的启动子甲基化和蛋白检测对评估胆囊癌浸润和转移具有重要意义。
Objective: To investigate the clinical significance of methylation status and protein expression of mismatch repair gene hMLH1 promoter in sporadic gallbladder carcinoma. Methods: Forty-seven patients with gallbladder cancer were collected. The methylation status and protein expression of hMLH1 promoter in gallbladder carcinoma were detected by methylation PCR and Envision immunohistochemistry. Results: The positive rate of hMLH1 promoter methylation was significantly different between liver infiltration group, lymph node metastasis group and Nevin staging (P <0.01 or P <0.05). hMLH1 protein expression was significantly different between hMLH1 gene promoter group, liver infiltration group, lymph node metastasis group and Nevin staging (P <0.01 or P <0.05). CONCLUSION: Promoter methylation in sporadic gallbladder carcinoma is one of the causes of inactivation of hMLH1 gene. The promoter methylation and protein detection of hMLH1 is of great significance for evaluating the infiltration and metastasis of gallbladder carcinoma.