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近十年来,由于膜片钳技术(Patch Clamp)的广泛应用,对游离单个心肌细胞和小片膜单个离子通道电活动的研究日益深入,进入了细胞和分子水平,概念不断更新,新的离子通道不断发现,例如目前确知的心肌细胞钙通道至少有两种,钾通道至少有八种,从而使人们对心肌细胞电活动的发生原理有了进一步的认识。另一方面,心肌的细胞、分子电生理学和分子生物学的结合,使人们有可能对离子通道蛋白内部结构和功能关系进行研究,包括通道的选择性通透性、激活门、失活门的定位研究等,促进了膜生物物理学的发展。
In recent ten years, due to the extensive use of patch clamp technology, the study on the electrical activity of individual ion channels of free single cardiomyocytes and small pieces of membranes has deepened into the cell and molecular level, the concepts are continuously updated, and new ion channels It has been found that there are at least two types of calcium channels in cardiomyocytes and at least eight types of potassium channels in cardiac cells so that people can further understand the principle of the occurrence of cardiomyocyte electrical activity. On the other hand, the combination of myocardial cells, molecular electrophysiology and molecular biology makes it possible to study the internal structure and function of ion channel proteins, including the selective permeability of channels, the activation of the gates, the inactivation of the gates Positioning research, etc., to promote the development of membrane biophysics.