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目的:为了探索肾癌增殖细胞的生物学特性与预后的关系.方法:应用免疫组化ABC法和流式细胞术DNA分析技术.结果:检测42例肾癌PCNA阳性率(LI)平均为12.4±5.1%.其中二倍体18例,LI值为2.41±3.14%;异倍体24例,LI值为6.80±4.29%.以LI均值为界分组,A组LI值≤12.4%,有23例,5年生存率为60.9%(14/23);B组LI值>12.4%,有19例,5年生存率为15.8%(3/19).两组差异非常显著(P<0.01).结论:提示PCNA的LI值可作为判断恶性度的客观指标,DNA直方图可评估预后.
Objective: To explore the relationship between the biological characteristics of proliferating cells and prognosis of renal cell carcinoma.Methods: The immunohistochemical ABC method and flow cytometry DNA analysis technique were used.Results: The positive rate of PCNA in 42 cases of renal cell carcinoma was 12.4 ± 5.1% .The diploid in 18 cases, LI value was 2.41 ± 3.14%; aneuploidy in 24 cases, LI value was 6.80 ± 4.29% .LIM mean value for the grouping, A group LI value ≤ 12.4%, 23 The 5-year survival rate was 60.9% (14/23), the LI value was 12.4% in group B, 19 cases and 15.8% (3/19) in group B. The difference between the two groups was significant (P <0.01) .Conclusion: It is suggested that the LI value of PCNA can be used as an objective index to judge the malignant degree, and the DNA histogram can evaluate the prognosis.