目的利用重组抗原BCOADA-E2和PDC-E2的二联体(BP),检测PBC患者血清中的M2抗体并探讨其临床意义。方法经Ni-NTA亲和柱纯化重组表达的BP融合蛋白,分别建立免疫印迹法(IBT)和酶链免疫吸附(ELISA)法检测60份PBC患者血清,以60份其他肝病患者6、0份自身免疫病患者8、0例正常人血清为对照组。结果经常规试剂盒检测为M2(+)的60份患者血清,利用重组抗原检测阳性53例,阴性7例,阳性率为88.3%;经常规试剂盒检测为M2(-)的60份自身免疫病患者血清、60份其他肝病患者和80例正常人血清,利用重组抗原检测为M2(-)。结论利用重组抗原BP检测M2抗体敏感性较高。对临床辅助诊断PBC提供一定的手段。 Objective To detect M2 antibody in serum of PBC patients and to explore its clinical significance by using duplexes of recombinant BCOADA-E2 and PDC-E2. Methods Recombinant BP fusion protein was purified by Ni-NTA affinity column. The serums of 60 patients with PBC were detected by Western blotting and enzyme-linked immunosorbent assay (ELISA). Sixty 8,0 patients with autoimmune disease normal serum as the control group. Results Sixty serum samples from 60 patients with M2 (+) were detected by routine kit, and 53 cases were detected by recombinant antigen and 7 cases were negative, the positive rate was 88.3%. 60 autoimmunity Patient sera, 60 other liver disease patients and 80 normal human sera were tested for M2 (-) using recombinant antigens. Conclusion The detection of M2 antibody using recombinant antigen BP is sensitive. Clinical support for the diagnosis of PBC provide a means.