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1990~1992年,从石河子、昌吉、阜康、乌鲁木齐及哈密等地采集了不同症状的辣椒病毒病标样75份。通过指示植物3次单斑分离,纯化后获8个病毒分离物,编号分别为P—91—5、P—91—7、P—91—8、P—91—10、P—89—2、P—89—27、P—90—3及P—90—4。上述分离物经病毒的寄生范围、鉴别寄主反应、传毒介体及传播方式、体外稳定性、电镜粒体观察、血清学反应及理化性质的测定,鉴定出6种病毒:黄瓜花叶病毒(CMV)占28%、烟草花叶病毒(TMV)占24.7%、蚕豆萎蔫病毒(BBWV)占5.0%、马铃薯X病毒(PVX)占4.3%、马铃薯Y病毒(PVY)占2.7%、烟草花叶病毒组一新成员病毒(暂定为C_aMV)占21.3%。还有两种未知病毒分别占13.2%及3%。研究表明,在北疆地区辣椒病毒病的主要毒源是CMV、TMV及C_aMV,但对辣椒危害性最大的是TMV及BBWV,导致辣椒萎蔫、坏死及顶枯。
From 1990 to 1992, 75 samples of pepper virus disease with different symptoms were collected from Shihezi, Changji, Fukang, Urumqi and Hami. Eight virus isolates were obtained after 3 single spot isolations of plants and were purified and identified as P-91-5, P-91-7, P-91-8, P-91-10 and P-89-2 , P-89-27, P-90-3 and P-90-4. The above isolates were identified 6 viruses by the parasitic range of the virus, the host reaction identification, the mediator and transmission mode of transmission, the stability in vitro, the observation by electron microscopy, the serological reaction and the physicochemical properties, and the cucumber mosaic virus CMV accounted for 28%, tobacco mosaic virus (TMV) accounted for 24.7%, BBWV accounted for 5.0%, potato X virus (PVX) accounted for 4.3%, potato virus Y (PVY) accounted for 2.7% A new member of the virus group (tentatively C_aMV) accounted for 21.3%. There are two unknown viruses accounted for 13.2% and 3%. Studies have shown that the main virulence of pepper virus disease in northern Xinjiang are CMV, TMV and C_aMV, but TMV and BBWV are the most harmful to pepper, resulting in wilting, necrosis and top wilt of pepper.