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用聚合酶链反应(PCR)可从杂交瘤和B细胞扩增得到Ig可变区(V)基因,并将其克隆到表达载体中,其后可筛选具结合活性的由细菌分泌的可溶抗体片段。如果在噬菌体表面表达抗体,那么V基因的筛选就方便得多,使用抗原可直接选择携带
The Ig variable region (V) gene can be amplified from hybridomas and B cells by polymerase chain reaction (PCR) and cloned into an expression vector whereupon it can be screened for soluble activity secreted by bacteria Antibody fragments. If the expression of antibodies on the surface of the phage, V gene screening is much more convenient, the use of antigen can be directly selected to carry