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目的:探讨米非司酮对早孕绒毛组织Oct4、Sox2、Nanog mRNA和蛋白水平表达的影响。方法:要求终止妊娠的正常早孕妇女60例,分为:负压吸宫组及服用米非司酮150mg及米索前列醇联合行药物流产组。运用Real-time PCR方法检测两组早孕绒毛组织中Oct4、Sox2、Nanog mRNA的表达;采用免疫组织化学方法检测两组早孕绒毛组织Oct4、Sox2、Nanog蛋白的定位及半定量表达情况,比较两组差异。结果:Oct4、Nanog、Sox2 mRNA在药物流产组早孕绒毛中相对表达量明显低于负压吸宫组,差异有统计学意义(相对表达量分别为:0.15±0.045;0.37±0.053;0.23±0.040,P值均<0.05);Oct4、Nanog、Sox2在药物流产组早孕绒毛蛋白表达量亦明显低于负压吸宫组,差异有统计学意义(药物流产组蛋白表达量分别为13869±541、19251±1503、139492±918明显低于负压吸宫组22017±235、30543±729、37237±710)。结论:米非司酮可以通过抑制早孕绒毛中Oct4、Sox2、Nanog表达,发挥抗早孕作用。
Objective: To investigate the effect of mifepristone on the expression of Oct4, Sox2, Nanog mRNA and protein in villus of early pregnancy. Methods: Sixty normal pregnant women requiring termination of pregnancy were divided into three groups: negative pressure inhalation group and taking mifepristone 150mg and misoprostol combined with medical abortion group. Real-time PCR method was used to detect the expression of Oct4, Sox2, Nanog mRNA in the first trimester villi of the two groups. Immunohistochemistry was used to detect the localization and semi-quantitative expression of Oct4, Sox2 and Nanog protein in the first trimester villi of the two groups. difference. Results: The relative expression levels of Oct4, Nanog and Sox2 mRNA in the abortion group were significantly lower than those in the negative pressure group (the relative expression levels were 0.15 ± 0.045, 0.37 ± 0.053 and 0.23 ± 0.040, respectively) , P <0.05). The expression of Oct4, Nanog and Sox2 in the first trimester of medical abortion group was also significantly lower than that in the negative pressure group (the difference was statistically significant (the protein expression levels in the abortion group were 13869 ± 541, 19251 ± 1503,139492 ± 918 was significantly lower than the negative pressure suction group 22017 ± 235,30543 ± 729,37237 ± 710). Conclusion: Mifepristone can play an anti-pregnancy effect by inhibiting the expression of Oct4, Sox2 and Nanog in early villus.