NAC domain proteins are plant-specific transcription factors known to play diverse roles in various plant developmental processes.In the present study,we performed the first comprehensive study of the NAC gene family in Gossypium raimondii Ulbr.,incorporating phylogenetic,chromosomal location,gene structure,conserved motif,and expression profiling analyses.We identified 145 NAC transcription factor (NAC-TF) genes that were phylogenetically clustered into 18 distinct subfamilies.Of these,127 NAC-TF genes were distributed across the 13 chromosomes,80(55%) were preferentially retained duplicates located in both duplicated regions and six were located in triplicated chromosomal regions.The majority of NAC-TF genes showed temporal-,spatial-,and tissue-specific expression patterns based on transcriptomic and qRT-PCR analyses.However,the expression patterns of several duplicate genes were partially redundant,suggesting the occurrence of sub-functionalization during their evolution.Based on their genomic organization,we concluded that genomic duplications contributed significantly to the expansion of the NAC-TF gene family in G.raimondii.Comprehensive analysis of their expression profiles could provide novel insights into the functional divergence among members of the NAC gene family in G.raimondii. NAC domain proteins are plant-specific transcription factors known to play diverse roles in various plant developmental processes. In the present study, we performed the first comprehensive study of the NAC gene family in Gossypium raimondii Ulbr., Incorporating phylogenetic, chromosomal location, gene structure , conserved motif, and expression profiling analyzes. We identified 145 NAC transcription factor (NAC-TF) genes that were phylogenetically clustered into 18 distinct subfamilies. Of these 127 NAC- TF genes were distributed across the 13 chromosomes, 80 were preferentially retained duplicates located both in duplicated regions and six were located in triplicated chromosomal regions. the majority of NAC-TF genes showed temporal-, spatial-, and tissue-specific expression patterns based on transcriptomic and qRT-PCR analyzes. expression patterns of several duplicate genes were partially redundant, suggesting the occurrence of sub-functionalization during their evolution eir genomic organization, we said that genomic duplications contributed significantly to the expansion of the NAC-TF gene family in G. raimondii. Comprehensive understanding of their expression profiles could provide novel insights into the functional divergence among members of the NAC gene family in G. raimondii.