从高粱(SorghumbicolorL.var.R111)幼苗中提取总RNA,利用RT-PCR和cDNA的3’末端的快速扩增方法(3’RACE),第一次克隆了高粱隐花色素2基因(CRY2)的cDNA序列。该序列包括了一个完整的开放阅读框,编码大小为690个氨基酸残基的蛋白质,与水稻、番茄和拟南芥CRY2蛋白质的同源性分别为87%、57%和45.5%。高粱CRY2基因组DNA含有3个内含子和4个外显子。RT-PCR检测结果表明,高粱CRY2基因在根、茎和叶中都有转录。Westernblotting结果显示CRY2蛋白在根、茎和叶中表达,并在黑暗中积累,蓝光下降解。高粱CRY2可能在蓝光诱导的幼苗去黄化反应中起作用。 Total RNA was extracted from sorghum (SorghumbicolorL.var.R111) seedlings. Sorghum cryptochrome 2 (CRY2) gene was cloned for the first time by RT-PCR and rapid amplification of 3 ’end of cDNA (3’RACE) CDNA sequence. The sequence contains a complete open reading frame (ORF) encoding a protein of 690 amino acid residues with homology of 87%, 57% and 45.5% to the CRY2 protein of rice, tomato and Arabidopsis, respectively. Sorghum CRY2 genomic DNA contains 3 introns and 4 exons. The results of RT-PCR indicated that the sorghum CRY2 gene transcribed in roots, stems and leaves. Western blotting results showed that CRY2 protein was expressed in roots, stems and leaves, accumulated in darkness and degraded in blue light. Sorghum CRY2 may play a role in the blue light-induced de-etiolation of seedlings.