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鼻咽癌对我国南部居民的健康造成严重的威胁.为了研究鼻咽癌的发病机理,本研究采用了蛋白质组学技术分析和比较了鼻咽癌细胞系(HNE1和CNE1)与永生化的鼻咽上皮细胞系的蛋白质表达谱.采用双向凝胶电泳分离提取的全细胞蛋白质,通过PDQuest软件分析找出在肿瘤中表达变化的蛋白质点,用基质辅助激光解析电离飞行时间串联质谱(MALDI-TOF/TOF-MS)进行鉴定.共得到了15个在肿瘤细胞系中表达上调和18个在肿瘤细胞系中表达下调的蛋白质,并对其中一些蛋白质的表达进行免疫印迹的验证.这些表达差异的蛋白质与细胞的增殖和调亡、癌症的转移,细胞骨架,信号传导等有关.本研究鉴定了一批可能作为鼻咽癌治疗的药物靶标的蛋白质,并对研究鼻咽癌发病机理提供了相关的线索.
In order to study the pathogenesis of nasopharyngeal carcinoma, proteomics technology was used to analyze and compare nasopharyngeal carcinoma cell lines (HNE1 and CNE1) with immortalized nasal The protein expression profile of pharyngeal epithelial cell line was analyzed by using two-dimensional gel electrophoresis (SDS-PAGE). The protein spots were detected by PDQuest software and analyzed by matrix-assisted laser desorption / ionization time of flight tandem mass spectrometry (MALDI-TOF / TOF-MS) .A total of 15 proteins that were upregulated in tumor cell lines and 18 down-regulated in tumor cell lines were obtained and some of these proteins were verified by immunoblotting Protein and cell proliferation and apoptosis, cancer metastasis, cytoskeleton, signal transduction, etc .. This study identified a number of possible as a drug target for the treatment of nasopharyngeal carcinoma protein, and to study the pathogenesis of nasopharyngeal carcinoma provide a correlation The clues.