论文部分内容阅读
[目的]探讨PARK7/DJ-1的突变与帕金森病的关系。[方法]以免疫共沉淀和免疫荧光的实验方法首次对DJ-1与MAP8可能存在关系,DJ-1与MAP8在细胞中存在共定位的作用关系进行探讨。[结果]DJ-1是一种在哺乳动物组织中广泛分布的蛋白,它可以保护细胞对抗氧化应激损伤以及具有分子伴侣的功能。细胞骨架系统的异常和神经变性疾病的发生密切相关,多种微管相关蛋白的非正常聚集可以引起神经变性疾病。MAP8是一种微管相关蛋白,在神经元形态发生和神经轴突生长中具有重要作用。实验进一步证明DJ-1可以抑制MAP8的非正常聚集和内含体(inclu-sion body)的形成。[结论]帕金森病是一种常见的神经变性疾病,PARK7/DJ-1的突变可以引起早发性的帕金森症状。
[Objective] To investigate the relationship between the mutation of PARK7 / DJ-1 and Parkinson’s disease. [Method] For the first time, the relationship between DJ-1 and MAP8 may be related to the method of co-immunoprecipitation and immunofluorescence. The interaction between DJ-1 and MAP8 in colorectal cancer cells is discussed. [Result] DJ-1 is a widely distributed protein in mammalian tissues. It can protect cells against oxidative stress and has molecular chaperone function. Abnormal cytoskeletal system and neurodegenerative diseases are closely related to the abnormal accumulation of a variety of microtubule-associated proteins can cause neurodegenerative diseases. MAP8 is a microtubule-associated protein that plays an important role in neuronal morphogenesis and axonal growth. The experiment further proves that DJ-1 can inhibit the abnormal aggregation of MAP8 and the formation of inclusions body. [Conclusion] Parkinson ’s disease is a common neurodegenerative disease. The mutation of PARK7 / DJ-1 can cause premature Parkinson’ s disease.