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AIM:To clone and identify genes differentially expressed in the EB virus transformed human B cell.METHODS:Suppression subtractive hybridization was used to construct the library which contains the differentiately expressed cDNAs in EB virus transformed human B cell.Then the isolated genes were cloned and sequenced and identifed by RT PCR.Nucleic acid homology searches were performed using the BLAST program.RESULTS:By this technique,4 differentiately expressed gene cDNA fragments of EB virus transformed human B cell were obtained.CONCLUSION:SSH is an effective method to isolate differentiately expressed genes.
AIM: To clone and identify genes differentially expressed in the EB virus transformed human B cell. METHODS: Suppression subtractive hybridization was used to construct the library which contains the differentially expressed cDNAs in EB virus were transformed human B cell. The the derived genes were cloned and sequenced and identifed by RT PCR. Nucleic acid homology searches were performed using the BLAST program .RESULTS: By this technique, 4 differentiately expressed gene fragments fragments of EB virus were obtained.CONCLUSION: SSH is an effective method to isolate differentiately expressed genes.