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目的:探讨大鼠腹部开放伤合并海水浸泡对血浆内毒素水平的影响。方法:将50只成年健康Wistar大鼠随机分为5组,每组10只。A组为正常对照组,B组为单纯腹部开放伤组,C组为单纯腹部海水浸泡组,D组为腹部开放伤合并生理盐水浸泡组,E组为腹部开放伤合并海水浸泡组。建立大鼠腹部开放伤合并海水浸泡创伤模型。每组取大鼠全血分离出血浆,应用MB-80微生物动态快速测定系统测定大鼠血浆内毒素含量。结果:腹部开放伤合并海水浸泡组大鼠血浆内毒素显著高于正常对照组(P<0.01),且明显高于单纯腹部海水浸泡组、单纯腹部开放伤组和腹部开放伤合并生理盐水浸泡组(P<0.01)。结论:大鼠腹部开放伤合并海水浸泡后肠黏膜屏障功能受损,血浆内毒素含量明显升高。
Objective: To investigate the effect of open abdomen injury and seawater immersion on plasma endotoxin in rats. Methods: Fifty healthy adult Wistar rats were randomly divided into 5 groups with 10 rats in each group. Group A was normal control group, group B was simple abdominal open injury group, group C was simple abdominal seawater immersion group, group D was abdominal open injury combined with saline immersion group, group E was open abdominal wound and seawater immersion group. Establishment of a rat model of open abdomen injury and seawater immersion wounds. Blood plasma was separated from whole rat blood in each group, and plasma endotoxin level in rats was determined by MB-80 rapid dynamic system. Results: The level of plasma endotoxin in open abdominal wound and seawater immersion group was significantly higher than that in normal control group (P <0.01), and significantly higher than that in simple abdominal seawater immersion group, open abdominal wound group and open abdominal wound combined with saline immersion group (P <0.01). CONCLUSION: The function of intestinal mucosal barrier is impaired after open abdomen injury and seawater immersion in rats, and the content of endotoxin in plasma is significantly increased.