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[目的]探讨神经肽Y(NPY)对实验性变态反应性脑脊髓炎(EAE)发病及血清Th2细胞因子IL-4含量的影响,研究NPY对EAE发病的作用及其可能的免疫机制。[方法]30只豚鼠随机分为正常对照组、EAE对照组、NPY干预组(侧脑室注射NPY)。观察EAE对照组、NPY干预组的发病潜伏期、神经功能障碍评分情况及3个组豚鼠血清IL-4的含量,并分析他们之间的相关性。[结果]与EAE对照组相比较,NPY干预组发病潜伏期明显延长(P﹤0.001),高峰期神经功能障碍评分明显降低(P﹤0.001),血清IL-4含量明显升高(P﹤0.001)。[结论]实验性变态反应性脑脊髓炎豚鼠进行侧脑室注射NPY干预能促进Th2细胞因子IL-4分泌,对EAE发病能起到保护作用,延长发病潜伏期并减轻神经功能障碍。
[Objective] To investigate the effect of neuropeptide Y (NPY) on the pathogenesis of experimental allergic encephalomyelitis (EAE) and the level of serum Th2 cytokine IL-4, and to investigate the effect of NPY on the pathogenesis of EAE and its possible immune mechanism. [Method] Thirty guinea pigs were randomly divided into normal control group, EAE control group and NPY intervention group (intracerebroventricular injection of NPY). Observe EAE control group, NPY intervention group latency incidence, neurological dysfunction scores and the three groups of guinea pig serum IL-4 content, and analyze the correlation between them. [Results] Compared with EAE control group, the incubation period of NPY intervention group was significantly longer (P <0.001), the peak of neurological deficit was significantly lower (P <0.001) and the level of serum IL-4 was significantly higher (P <0.001) . [Conclusion] NPY intervention in experimental allergic encephalomyelitis guinea pigs can promote the secretion of Th2 cytokine IL-4, play a protective role in the pathogenesis of EAE, prolong the incubation period and reduce neurological dysfunction.