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目的 了解脊柱关节病 (SpA)患者外周血和关节液单个核细胞 (SFMC)基因的变化及其特点 ,探讨与SpA关节炎相关的基因及可能的意义。 方法 采用含 1176个基因的cDNA微阵列 ,检测 6名健康志愿者外周血单个核细胞 (PBMC) ,5例SpA和 5例类风湿关节炎 (RA)病人PBMC和关节液SFMC的基因表达 ,挑选SpASFMC表达异常的 9个致炎、抗炎、信号传导基因或受体和粘附分子 ,扩大病例数以半定量PCR再验证微阵列检测结果。结果 cDNA微阵列和PCR结果显示 ,1176cDNA微阵列基因图谱和阳性基因数量在RA和SpA病人SFMC组无明显区别 ,比较SpA或RA病人的SFMC和PBMC ,发现SpA和RA的SFMC中的阳性基因均少于各自的PBMC。在RA的PBMC有 5 3个基因明显高于RASFMC (P <0 0 5 ) ,但在SpAPBMC仅有 5个基因明显高于SpASFMC (P <0 0 5 )。SpA病人SFMC的IL 1β、TNF α、TGF β、TGF β2 、c jun、JAK 3显著高于健康人PBMC (P <0 0 5 )。结论 SpA患者的SFMC基因表达谱异常 ,但与RA的SFMC未见明显特征型区别 ,IL 1β、TNF α、TGF β、TGF β2 、c jun、JAK 3与SpA关节炎的发生和发展相关。
Objective To investigate the changes and characteristics of peripheral blood and synovial fluid mononuclear cells (SFMC) in patients with spondyloarthropathies (SpA) and to explore the possible significance of the genes involved in SpA arthritis. Methods cDNA microarrays containing 1176 genes were used to detect the gene expression of peripheral blood mononuclear cells (PBMCs) from 6 healthy volunteers, PBMC from 5 SpA and 5 rheumatoid arthritis (RA) patients, and SFMC from synovial fluid Nine inflammatory, anti-inflammatory, and signal transducing genes or receptors and aberrant adhesion molecules were expressed in SpASFMC, and the number of cases was expanded to retest the microarray results by semi-quantitative PCR. Results The results of cDNA microarray and PCR showed that there was no significant difference between the 1176 cDNA microarray gene and the number of positive genes in the SFMC of patients with RA and SpA. SFMC and PBMC of patients with SpA or RA were compared. Less than the respective PBMC. There were 53 genes in PBMC of RA that were significantly higher than that of RASFMC (P <0.05), but only 5 genes in SpAPBMC were significantly higher than those in SpASFMC (P <0.05). The levels of IL-1β, TNFα, TGFβ, TGFβ2, c jun and JAK 3 in SFMC of SpA patients were significantly higher than that of healthy PBMCs (P <0.05). Conclusion The expression of SFMC in SpA patients is abnormal, but there is no significant difference between the two groups in SFMC. The incidence of IL-1β, TNFα, TGFβ, TGFβ2, c jun, JAK 3 are correlated with the occurrence and development of SpA.