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[目的]探讨乙型肝炎病毒(HBV)感染人群HLA-DR基因、HBV基因型与肝细胞癌的相关性。[方法]2007~2008年,应用序列特异性引物-聚合酶链反应(SSP-PCR)方法与荧光PCR(探针)法,分别对40例慢性乙肝患者(试验1组)、38例肝硬化患者(试验2组)和34例肝细胞癌患者(试验3组)的HLA-DR等位基因、HBV基因型进行检测,并以中华骨髓库山东分库无血缘关系的自愿骨髓捐献者1 383例为对照,分析其表达与肝细胞癌的相关性。[结果]HLA-DR1基因的检出率,试验3组为14.71%,对照组为4.85%(P<0.05);HLA-DR13基因的检出率,试验3组为14.71%,试验1组为0.00%(P<0.05);试验2组与试验3组各等位基因表型的检出率差异均无统计学意义(P>0.05)。HBV基因型B型、C型、非B非C型所占比例,试验1、2、3组的差异无统计学意义(P>0.05)。试验3组中,HLA-DR1、DR13阳性的5例均为HBV基因C型;其他29例HBV基因C型23例、非B非C型6例(P>0.05)。[结论]HLA-DR1可能是肝细胞癌的易感基因,携带HLA-DR13基因位点的HBV感染者更易发展为肝细胞癌。
[Objective] To investigate the correlation between HLA-DR and HBV genotypes and hepatocellular carcinoma in Hepatitis B virus (HBV) infected population. [Methods] From 2007 to 2008, 40 patients with chronic hepatitis B (test group 1) and 38 patients with cirrhosis (cirrhosis) were enrolled in this study by using sequence specific primer - polymerase chain reaction (SSP - PCR) HLA-DR alleles and HBV genotypes in patients (group 2) and 34 patients with hepatocellular carcinoma (group 3) were tested by a voluntary bone marrow donor unrelated to the China Marrow Bank Shandong Branch Cases as a control, analyze its expression and hepatocellular carcinoma correlation. [Results] The detection rate of HLA-DR1 gene was 14.71% in test group 3 and 4.85% in control group (P <0.05). The detection rate of HLA-DR13 gene in test group 3 was 14.71% 0.00% (P <0.05). There was no significant difference in the phenotypes of alleles between experimental group 2 and experimental group 3 (P> 0.05). HBV genotype B, C, non-B non-C proportion of the proportion of test 1,2,3 group no significant difference (P> 0.05). In group 3, HLA-DR1 and DR13 were all positive for HBV genotype C in all 5 cases. Twenty-nine other HBV genotypes were C genotype in 23 cases and non-B non-C genotype in 6 cases (P> 0.05). [Conclusion] HLA-DR1 may be a susceptible gene of hepatocellular carcinoma. HBV carriers carrying HLA-DR13 locus are more likely to develop hepatocellular carcinoma.