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非豆科固氮树木和豆科植物一样,同属于共生固氮体系,它们的固氮量远远高于自生固氮微生物。因此,历来受到人们的重视。然而,非豆科固氮树种根瘤内生菌的分离和纯培养一直未能得到解决,严重地阻碍了这个领域的基础理论研究和在林业生产上的应用。经过近70年的努力,1978年美国才首次从香蕨木根瘤中分离得固氮放线菌Frankia菌株。但到目前为止,仅从香蕨木、赤杨、胡颓子、沙棘、杨梅等属的根瘤中分离得内生菌,还有许多属宿主植物的根瘤内生菌至今还未能分离出来。最近(1982)H. G Diem等在加拿大微生物学杂志发表了分离木麻黄根瘤内生菌的报告,尽管他们实验证明分离菌确实属于弗
Non-legume nitrogen-fixing trees and legumes, like the same symbiotic nitrogen fixation system, their nitrogen fixation is much higher than the autotrophic nitrogen fixation microorganisms. Therefore, it has always been people’s attention. However, the isolation and pure culture of non-legume nitrogen-fixing root nodule endophytes has not been solved, which seriously hindered the basic theoretical research in this field and its application in forestry production. After nearly 70 years of efforts, the United States for the first time in 1978 from the fragrant wood nodules isolated Actinomyces Frankia strains. So far, however, endophytes have been isolated from root nodules of the genus Bracken, Alnus cremastogyne, Elaeagnus, Hippophae rhamnoides and Myrica rubra, and many endophyte endophytes belonging to host plants have not yet been isolated. Recently (1982) H. G Diem et al published in the Journal of Microbiology, Canada, the report of the separation of endotoxins from the genus Casuarina, although they have experimentally proved that the isolates belong to the E